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phospho thr642 akt substrate  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phospho thr642 akt substrate
    Phospho Thr642 Akt Substrate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+thr642+akt+substrate/Phospho-AS160+(Thr642)+Antibody/10__1113_slash_jp289864-102-72-79
    Average 95 stars, based on 229 article reviews
    phospho thr642 akt substrate - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Saline:

    Article Title: Isolating the effects of carbohydrate and lipid availability on exercise‐induced skeletal muscle signalling in males
    Article Snippet: An equal amount of protein (50 μg) was loaded into each well in 10% Tris-glycine SDS-polyacrylamide gels and proteins were separated using SDS-PAGE at 200 V. Proteins were transferred to a nitrocellulose membrane via semi-dry electro-blotting apparatus (Bio-Rad, Hercules, CA,USA). .. Themembranes were washed in Tris-buffered saline (0.09% NaCl, 100 mm Tris-HCl pH 7.4) with 0.1% Tween 20 (TBS-T) and incubated for 30 min in a blocking solution of 5% non-fat dried milk (Marvel; Premier Foods, Dublin, Ireland) in TBS-T. Membranes were washed again in TBS-T and incubated in primary antibodies against phospho Ser 79 acetyl-CoA carboxlyase (ACC) (Cell Signaling Technologies, Danvers, MA, USA), phospho Thr172 AMP-dependent protein kinase (AMPK) (Cell Signaling Technologies), phospho-Thr642 Akt substrate of 160 kDa (AS160) (Cell Signaling Technologies), phospho-Tyr1150/1151 insulin receptor (INS) (Cell Signaling Technologies), phospho-Ser 473 protein kinase B (Akt) (Cell Signaling Technologie), phospho-Ser660 hormone-sensitive lipase (HSL) (Cell Signaling Technologies), phospho-Ser 293 pyruvate dehydrogenase (PDH) (Cell Signaling Technologies), vinculin (Cell Signaling Technologies), pyruvate dehydrogenase kinase 4 (PDK4) (Abgent, San Diego, CA, USA) and beta-actin (Protein Technologies, Stockport, UK) overnight at 4°C. ..

    Incubation:

    Article Title: Isolating the effects of carbohydrate and lipid availability on exercise‐induced skeletal muscle signalling in males
    Article Snippet: An equal amount of protein (50 μg) was loaded into each well in 10% Tris-glycine SDS-polyacrylamide gels and proteins were separated using SDS-PAGE at 200 V. Proteins were transferred to a nitrocellulose membrane via semi-dry electro-blotting apparatus (Bio-Rad, Hercules, CA,USA). .. Themembranes were washed in Tris-buffered saline (0.09% NaCl, 100 mm Tris-HCl pH 7.4) with 0.1% Tween 20 (TBS-T) and incubated for 30 min in a blocking solution of 5% non-fat dried milk (Marvel; Premier Foods, Dublin, Ireland) in TBS-T. Membranes were washed again in TBS-T and incubated in primary antibodies against phospho Ser 79 acetyl-CoA carboxlyase (ACC) (Cell Signaling Technologies, Danvers, MA, USA), phospho Thr172 AMP-dependent protein kinase (AMPK) (Cell Signaling Technologies), phospho-Thr642 Akt substrate of 160 kDa (AS160) (Cell Signaling Technologies), phospho-Tyr1150/1151 insulin receptor (INS) (Cell Signaling Technologies), phospho-Ser 473 protein kinase B (Akt) (Cell Signaling Technologie), phospho-Ser660 hormone-sensitive lipase (HSL) (Cell Signaling Technologies), phospho-Ser 293 pyruvate dehydrogenase (PDH) (Cell Signaling Technologies), vinculin (Cell Signaling Technologies), pyruvate dehydrogenase kinase 4 (PDK4) (Abgent, San Diego, CA, USA) and beta-actin (Protein Technologies, Stockport, UK) overnight at 4°C. ..

    Article Title: Isolating the effects of carbohydrate and lipid availability on exercise‐induced skeletal muscle signalling in males
    Article Snippet: .. Membranes were washed again in TBS‐T and incubated in primary antibodies against phospho Ser 79 acetyl‐CoA carboxlyase (ACC) (Cell Signaling Technologies, Danvers, MA, USA), phospho Thr172 AMP‐dependent protein kinase (AMPK) (Cell Signaling Technologies), phospho‐Thr642 Akt substrate of 160 kDa (AS160) (Cell Signaling Technologies), phospho‐Tyr1150/1151 insulin receptor (INS) (Cell Signaling Technologies), phospho‐Ser 473 protein kinase B (Akt) (Cell Signaling Technologie), phospho‐Ser660 hormone‐sensitive lipase (HSL) (Cell Signaling Technologies), phospho‐Ser 293 pyruvate dehydrogenase (PDH) (Cell Signaling Technologies), vinculin (Cell Signaling Technologies), pyruvate dehydrogenase kinase 4 (PDK4) (Abgent, San Diego, CA, USA) and beta‐actin (Protein Technologies, Stockport, UK) overnight at 4°C. ..

    Blocking Assay:

    Article Title: Isolating the effects of carbohydrate and lipid availability on exercise‐induced skeletal muscle signalling in males
    Article Snippet: An equal amount of protein (50 μg) was loaded into each well in 10% Tris-glycine SDS-polyacrylamide gels and proteins were separated using SDS-PAGE at 200 V. Proteins were transferred to a nitrocellulose membrane via semi-dry electro-blotting apparatus (Bio-Rad, Hercules, CA,USA). .. Themembranes were washed in Tris-buffered saline (0.09% NaCl, 100 mm Tris-HCl pH 7.4) with 0.1% Tween 20 (TBS-T) and incubated for 30 min in a blocking solution of 5% non-fat dried milk (Marvel; Premier Foods, Dublin, Ireland) in TBS-T. Membranes were washed again in TBS-T and incubated in primary antibodies against phospho Ser 79 acetyl-CoA carboxlyase (ACC) (Cell Signaling Technologies, Danvers, MA, USA), phospho Thr172 AMP-dependent protein kinase (AMPK) (Cell Signaling Technologies), phospho-Thr642 Akt substrate of 160 kDa (AS160) (Cell Signaling Technologies), phospho-Tyr1150/1151 insulin receptor (INS) (Cell Signaling Technologies), phospho-Ser 473 protein kinase B (Akt) (Cell Signaling Technologie), phospho-Ser660 hormone-sensitive lipase (HSL) (Cell Signaling Technologies), phospho-Ser 293 pyruvate dehydrogenase (PDH) (Cell Signaling Technologies), vinculin (Cell Signaling Technologies), pyruvate dehydrogenase kinase 4 (PDK4) (Abgent, San Diego, CA, USA) and beta-actin (Protein Technologies, Stockport, UK) overnight at 4°C. ..

    other:

    Article Title: N-3PUFA differentially modulate palmitate-induced lipotoxicity through alterations of its metabolism in C2C12 muscle cells.
    Article Snippet: a Institut National de la Recherche Agronomique, UMR1019 Nutrition Humaine, Laboratoire de Nutrition Humaine, Université d’Auvergne, CRNH, 58 rue Montalembert BP321, 63009 Clermont Ferrand CEDEX 1, France b Institut National de la Recherche Agronomique, UMR1019 Nutrition Humaine, Plateforme d’Exploration du Métabolisme, 63122 Saint-Genès-Champanelle, France c Institut Cochin, Département d'Endocrinologie,Métabolisme andDiabète, U1016 Inserm/UMR8104 CNRS/UMR-S8104, bâtiment Faculté, 3 étage, Salle 3012A, 24 rue du faubourg Saint Jacques, 75014 Paris, France d INRA, UMR 1397, Laboratoire CarMeN, Université Lyon 1, INSERM U1060, INSA de Lyon, Université Lyon-Sud Rockefeller et Charles Merieux, Lyon, France



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